RNA

Part:BBa_K3760007:Design

Designed by: Ha Buer   Group: iGEM21_LZU-CHINA   (2021-10-01)


RfxCas13d-hACE2-sgRNA3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sgRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target RNA sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas13 protein, and different Cas13 proteins have different DR sequences. And the spacer is determined by a specific target

Source

It does not come from genomic DNA. It is artificially synthesized.

References

Wessels HH, Méndez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE. Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16. PMID: 32518401; PMCID: PMC7294996.